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ePub Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual download

by Theodore Friedmann,John Rossi

ePub Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual download
Author:
Theodore Friedmann,John Rossi
ISBN13:
978-0879697648
ISBN:
0879697644
Language:
Publisher:
Cold Spring Harbor Laboratory Press; 1st edition (December 7, 2006)
Category:
Subcategory:
Biological Sciences
ePub file:
1408 kb
Fb2 file:
1957 kb
Other formats:
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Rating:
4.3
Votes:
323

Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material.

Theodore Friedmann, John J. Rossi

Theodore Friedmann, John J. Rossi. Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material.

Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells

Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells 6.

Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells 62. Mifepristone–inducible Gene Regulatory System 63. Dimerizer–mediated Regulation of Gene Expression 64. 65. 66. Specialized techniques of gene and vector delivery 67. Assembly of De Novo Bacterial Artificial Chromosome–based Human Artificial Chromosomes 68. Delivery of Naked DNA Using Hydrodynamic Injection Techniques 69. Nonviral Gene Transfer across the Blood–brain Barrier with Trojan Horse Liposomes 70. 71. 72. 73. TRANSGENIC APPROACHES 74.

Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual. Eds. T. Friedmann & J. Cold Spring Harbor Laboratory Press.

Gene Transfer; Delivery and Expression of DNA and RNA, A Laboratory Manual. Theodore Friedmann, John Rossi, ed.

Gene Transfer: Delivery and Expression of DNA And RNA, A Laboratory Manual, Friedmann Theodore. Understanding gene function and regulation requires rigorous testign in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA int. одробнее Купить за 30315 руб. Advanced analysis of gene expression microarray data, Zhang Aidong

Theodore Friedmann, John J. Gene transfer: delivery and expression of DNA and RNA : a laboratory manual. DNA pharmaceuticals: formulation and delivery in gene therapy, DNA vaccination and immunotherapy. Vinod Labhasetwar, Diandra L. Leslie-Pelecky. Biomedical applications of nanotechnology.

Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Genetic material must reach the nucleus of the host cell to induce gene expression.

11Gene Transfer: Delivery and Expression of DNA And RNA, A Laboratory Manual Friedmann Theodore (2006) Understanding gene function and regulation requires rigorous testign in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells an. Scion Publishing Ltd 30930 руб. 12Ruby's Gift (+ Audio CD) Murray Sue (2009) Jeremy and his mother are on holiday in San Francisco.

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
  • This book describes a series of protocols that offer ways to deliver genes into mammalian cells. About half the book describes viral techniques where a virus is used to do the transport and delivery. The other half describes non-viral methods. The book has some 75 different techniques, each described by a researcher who has been using/developing that method.

    Note that many of these techniques are brand new and still in the stage of being developed. Some of them are likely to prove useful, others will be of limited use, perhaps in a niche application or will simply lead to better techniques later on.

    Dr. Rossi (Bechmann Research Institute) and Dr. Friedmann (UCSD) serve as editors of the book. It is called a Laboratory Manual as the protocols are presented in a stepwise, 'cookbook' style.

    The book was published in early 2007 and should be considered an accurate reflection of the state of the art as of late in 2006.

  • I bought a used book from this source, and when i got it i couldn't believe how new it looked. It looked like nobody has even opened it. I tried buying books from other sources, but by far this is the best one.. I plan on buying the rest of my books from them. Thank You!!!!